Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


(Plasmid #113941)


This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 113941 Standard format: Plasmid sent in bacteria as agar stab 1 $85


  • Vector backbone
  • Backbone manufacturer
    ThermoFisher Scientific
  • Backbone size w/o insert (bp) 4064
  • Total vector size (bp) 5315
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    For inducing protein expression with arabinose, grow in DH10B cells.
  • Copy number
    High Copy


  • Gene/Insert name
  • Species
  • Insert Size (bp)
  • GenBank ID
  • Tags / Fusion Proteins
    • 6xHis (N terminal on insert)
    • Modified TorA MNNNDLFQASRRRFLAQLG[G to S]LT[V to D]AG[M to T]LGPSLLTPRRATAAQAATDAS (N terminal on backbone)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Robert Campbell, University of Alberta.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTorPE-jREX-GECO1 was a gift from Robert Campbell & Thomas Hughes (Addgene plasmid # 113941 ; ; RRID:Addgene_113941)
  • For your References section:

    Understanding the Fluorescence Change in Red Genetically Encoded Calcium Ion Indicators. Molina RS, Qian Y, Wu J, Shen Y, Campbell RE, Drobizhev M, Hughes TE. Biophys J. 2019 May 21;116(10):1873-1886. doi: 10.1016/j.bpj.2019.04.007. Epub 2019 Apr 13. 10.1016/j.bpj.2019.04.007 PubMed 31054773