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(Plasmid #115474)


Item Catalog # Description Quantity Price (USD)
Plasmid 115474 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Vector type
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
  • Alt name
  • Alt name
    Cellobiohydrolase 1
  • Species
    Trichoderma reesei
  • Insert Size (bp)
  • Mutation
    Introns have been removed
  • GenBank ID
  • Promoter Trichoderma reesei Enolase (Eno) promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Pac1 (not destroyed)
  • 3′ cloning site Xba1 (not destroyed)
  • 5′ sequencing primer ATCGCCCAGCTACCTACCTC
  • 3′ sequencing primer GACCTGCGACAGACAACCAA
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This Trichoderma reesei expression vector currently expresses native T. reesei Cel7A (CBH1), but this can readily be exchanged for other coding sequences. Prior to integration into T. reesei genome, use SbfI and XhoI to linearize the vector.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTrEno was a gift from Steve Decker (Addgene plasmid # 115474 ; ; RRID:Addgene_115474)
  • For your References section:

    A constitutive expression system for glycosyl hydrolase family 7 cellobiohydrolases in Hypocrea jecorina. Linger JG, Taylor LE 2nd, Baker JO, Vander Wall T, Hobdey SE, Podkaminer K, Himmel ME, Decker SR. Biotechnol Biofuels. 2015 Mar 18;8:45. doi: 10.1186/s13068-015-0230-2. eCollection 2015. 230 [pii] PubMed 25904982