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pET_BGL1
(Plasmid #101910)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 101910 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET16b
  • Backbone size w/o insert (bp) 5711
  • Total vector size (bp) 6999
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    β-glucosidase
  • Alt name
    bgl
  • Species
    Thermomicrobium roseum
  • Insert Size (bp)
    1359
  • GenBank ID
    WP_015922700.1
  • Promoter T7+LacO

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer ttcccctctagaaataattttgtttaactttaagaag
  • 3′ sequencing primer gcagccggatccCTAACC
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET_BGL1 was a gift from Steve Shih (Addgene plasmid # 101910 ; http://n2t.net/addgene:101910 ; RRID:Addgene_101910)
  • For your References section:

    An Automated Induction Microfluidics System for Synthetic Biology. Husser MC, Vo PQN, Sinha H, Ahmadi F, Shih SCC. ACS Synth Biol. 2018 Mar 16;7(3):933-944. doi: 10.1021/acssynbio.8b00025. Epub 2018 Mar 8. 10.1021/acssynbio.8b00025 PubMed 29516725