TagBFP2-INPP4BC842A-CAAX
(Plasmid
#116858)
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Purposeinactive plasma membrane targetted PI(3,4)P2 phosphatase
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Depositing Lab
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Depositing OrganizationUniversity of Pittsburgh (University, School of Medicine, and Cancer Institute)
Located in United States of America -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 116858 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 116858-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 116858-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepTagBFP2-C1
- Total vector size (bp) 7530
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameINPP4B
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SpeciesH. sapiens (human)
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Insert Size (bp)2772
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Mutationcatalytic cysteine 842 changed to alanine
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Entrez GeneINPP4B
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Tags
/ Fusion Proteins
- TagBFP2 (N terminal on insert)
- HRAS(172-189) (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV-For
- 3′ sequencing primer SV40-pArev
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/early/2018/09/06/410811 for bioRxiv preprint.
DNA (Catalog # 116858-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 116858-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TagBFP2-INPP4BC842A-CAAX was a gift from Gerry Hammond (Addgene plasmid # 116858 ; http://n2t.net/addgene:116858 ; RRID:Addgene_116858) -
For your References section:
A high-avidity biosensor reveals plasma membrane PI(3,4)P2 is predominantly a class I PI3K signaling product. Goulden BD, Pacheco J, Dull A, Zewe JP, Deiters A, Hammond GRV. J Cell Biol. 2019 Mar 4;218(3):1066-1079. doi: 10.1083/jcb.201809026. Epub 2018 Dec 27. 10.1083/jcb.201809026 PubMed 30591513
Map uploaded by the depositor.
