Purpose(Empty Backbone) Lentiviral transfer vector containing a multible cloning site
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||116877||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Backbone manufacturerJames Thomson Lab
- Backbone size (bp) 8507
Modifications to backbonedeleted EF1-alpha promotor, added a MCS
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
- Cloning method Restriction Enzyme
- 5′ sequencing primer MJA 424 5’TTGCGTGCCTTGAATTACTTCCACCT
- 3′ sequencing primer MJA 425 5’AATAAGGCCGGTGTGCGTTTGTCTAT (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
The psPAX2 packaging plasmid and pMD2.G envelope plasmid can be used with this vector.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMJA284= pSin-Pur-MCS-Puro was a gift from Marcos Alcocer (Addgene plasmid # 116877 ; http://n2t.net/addgene:116877 ; RRID:Addgene_116877)