PurposeOverexpression of mouse LIN28A
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||117057||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Entrez GeneLin28a (a.k.a. AL024421, Gm10299, Lin-28, Lin28, Tex17, lin-28A)
- Promoter hPGK promoter
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer hPGK-F (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
This plasmid was generated by Mario Leonardo Squadrito.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:LV-LIN28A was a gift from Michele De Palma (Addgene plasmid # 117057 ; http://n2t.net/addgene:117057 ; RRID:Addgene_117057)
For your References section:Suppression of microRNA activity amplifies IFN-gamma-induced macrophage activation and promotes anti-tumour immunity. Baer C, Squadrito ML, Laoui D, Thompson D, Hansen SK, Kiialainen A, Hoves S, Ries CH, Ooi CH, De Palma M. Nat Cell Biol. 2016 Jul;18(7):790-802. doi: 10.1038/ncb3371. Epub 2016 Jun 13. 10.1038/ncb3371 PubMed 27295554