Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pMD19_STARR-seq_plants
(Plasmid #117379)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 117379 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    T-Vector pMD19 (Simple)
  • Backbone manufacturer
    Clontech
  • Backbone size (bp) 6332
  • Modifications to backbone
    T-Vector pMD19 (Simple) was digested with EcoR V and inserts were ligated into the backbone.
  • Vector type
    Plant Expression
  • Promoter 35s core promoter
  • Tag / Fusion Protein
    • GFP derived from pMDC107 (N terminal on insert)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Resource Information

Depositor Comments

This plasmid is designed for transient transfection of protoplasts. It has only been validated in Zea mays mesophyll protoplasts. The region containing the ccdB suicide gene is dropped out by a double-digest with SacI and KpnI and the large fragment is recombined with the DNA insert library.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMD19_STARR-seq_plants was a gift from Xiaoyu Zhang (Addgene plasmid # 117379 ; http://n2t.net/addgene:117379 ; RRID:Addgene_117379)
  • For your References section:

    Widespread long-range cis-regulatory elements in the maize genome. Ricci WA, Lu Z, Ji L, Marand AP, Ethridge CL, Murphy NG, Noshay JM, Galli M, Mejia-Guerra MK, Colome-Tatche M, Johannes F, Rowley MJ, Corces VG, Zhai J, Scanlon MJ, Buckler ES, Gallavotti A, Springer NM, Schmitz RJ, Zhang X. Nat Plants. 2019 Nov 18. pii: 10.1038/s41477-019-0547-0. doi: 10.1038/s41477-019-0547-0. 10.1038/s41477-019-0547-0 PubMed 31740773