pcDNA1-Bystin
(Plasmid #11869)

• Depositing Lab: Michiko Fukuda
• Publication: Suzuki et al Proc Natl Acad Sci U S A. 1998 Apr 28. 95(9):5027-32.

Backbone

• Vector backbone: pcDNA1
• Backbone size w/o insert (bp): 4033
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Tetracycline, 100 & 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10/P3
• Growth instructions: Use HB10/P3 strain, MC1061/P3 causes mutations. Select transformant in LB plus tetracyclin (10 ug/ml), at 37C. (SupF)
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Bystin
• Alt name: BYSL
• Species: H. sapiens (human)
• Entrez Gene: BYSL (a.k.a. BYSTIN, Enp1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Sanford-Burnham Medical Research Institute Ancillary Agreement
• Industry Terms:
  • Not Available to Industry