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pcDNA1-Bystin
(Plasmid #11869)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 11869 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Tetracycline, 100 & 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10/P3
  • Growth instructions
    Use HB10/P3 strain, MC1061/P3 causes mutations. Select transformant in LB plus tetracyclin (10 ug/ml), at 37C. (SupF)
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Bystin
  • Alt name
    BYSL
  • Species
    H. sapiens (human)
  • Entrez Gene
    BYSL (a.k.a. BYSTIN, Enp1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA1-Bystin was a gift from Michiko Fukuda (Addgene plasmid # 11869 ; http://n2t.net/addgene:11869 ; RRID:Addgene_11869)
  • For your References section:

    A cytoplasmic protein, bystin, interacts with trophinin, tastin, and cytokeratin and may be involved in trophinin-mediated cell adhesion between trophoblast and endometrial epithelial cells. Suzuki N, Zara J, Sato T, Ong E, Bakhiet N, Oshima RG, Watson KL, Fukuda MN. Proc Natl Acad Sci U S A. 1998 Apr 28. 95(9):5027-32. 10.1073/pnas.95.9.5027 PubMed 9560222