MiniCoopR 2xU6:gRNA, mitfa:Cas9
Purpose(Empty Backbone) Targets 2 genes and expresses zebrafish mitfa specifically in zebrafish melanocytes
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||118844||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeCRISPR ; Tol2
Growth in Bacteria
Copy numberHigh Copy
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Use the BseRI enzyme to clone gRNA of interest in the first U6:gRNA cassette. Use primer: CCATACCACATTTGTAGAGGT to sequence insert. Use the aaRI enzyme to clone gRNA of interest in the second U6:gRNA cassette. Use primer: GCTTACAACAAACACAGAGA to sequence insert.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:MiniCoopR 2xU6:gRNA, mitfa:Cas9 was a gift from Leonard Zon (Addgene plasmid # 118844 ; http://n2t.net/addgene:118844 ; RRID:Addgene_118844)
For your References section:Human tumor genomics and zebrafish modeling identify SPRED1 loss as a driver of mucosal melanoma. Ablain J, Xu M, Rothschild H, Jordan RC, Mito JK, Daniels BH, Bell CF, Joseph NM, Wu H, Bastian BC, Zon LI, Yeh I. Science. 2018 Nov 1. pii: science.aau6509. doi: 10.1126/science.aau6509. 10.1126/science.aau6509 PubMed 30385465