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pLV-shEsr2
(Plasmid #120722)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 120722 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLL3.7
  • Backbone manufacturer
    MIT
  • Backbone size w/o insert (bp) 7649
  • Total vector size (bp) 7700
  • Vector type
    Mammalian Expression, Lentiviral, RNAi

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Esr2
  • gRNA/shRNA sequence
    gtacgaagacagagaagtg
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Esr2 (a.k.a. ER[b], ERbe, ERbeta, Est, Estrb)
  • Promoter mouse U6
  • Tag / Fusion Protein
    • GFP

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HpaI (destroyed during cloning)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer U6
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV-shEsr2 was a gift from Amy Lasek (Addgene plasmid # 120722 ; http://n2t.net/addgene:120722 ; RRID:Addgene_120722)
  • For your References section:

    Estrogen Receptor alpha Regulates Ethanol Excitation of Ventral Tegmental Area Neurons and Binge Drinking in Female Mice. Vandegrift BJ, Hilderbrand ER, Satta R, Tai R, He D, You C, Chen H, Xu P, Coles C, Brodie MS, Lasek AW. J Neurosci. 2020 Jul 1;40(27):5196-5207. doi: 10.1523/JNEUROSCI.2364-19.2020. Epub 2020 Jun 1. 10.1523/JNEUROSCI.2364-19.2020 PubMed 32482639