PurposeLentiviral vector that expresses GFP and an shRNA targeting Esr2 (in pLL3.7).
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||120722||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 7649
- Total vector size (bp) 7700
Vector typeMammalian Expression, Lentiviral, RNAi
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Entrez GeneEsr2 (a.k.a. ER[b], ERbeta, Estrb)
- Promoter mouse U6
/ Fusion Protein
- Cloning method Restriction Enzyme
- 5′ cloning site HpaI (destroyed during cloning)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer U6 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLV-shEsr2 was a gift from Amy Lasek (Addgene plasmid # 120722 ; http://n2t.net/addgene:120722 ; RRID:Addgene_120722)
For your References section:Estrogen Receptor alpha Regulates Ethanol Excitation of Ventral Tegmental Area Neurons and Binge Drinking in Female Mice. Vandegrift BJ, Hilderbrand ER, Satta R, Tai R, He D, You C, Chen H, Xu P, Coles C, Brodie MS, Lasek AW. J Neurosci. 2020 Jul 1;40(27):5196-5207. doi: 10.1523/JNEUROSCI.2364-19.2020. Epub 2020 Jun 1. 10.1523/JNEUROSCI.2364-19.2020 PubMed 32482639