p53 S23A targeting vector
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12167||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepBluescript II SK
- Backbone size w/o insert (bp) 3000
Vector typeMammalian Expression, Cre/Lox
Growth in Bacteria
Copy numberHigh Copy
Alt namep53 targeting vector
SpeciesM. musculus (mouse)
MutationSer23 mutation (S23A) in exon 2 and puromycin gene flanked by loxP sites in intron 1; vector includes p53 gene locus from intron 1 through exon 6.
Entrez GeneTrp53 (a.k.a. Tp53, bbl, bfy, bhy, p44, p53)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Used to generate a mouse with the endogenous p53 serine 23 mutated. Puromycin cassette flanked by loxP sites is in the reverse orientation and contains an EcoR1 site.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p53 S23A targeting vector was a gift from Tyler Jacks (Addgene plasmid # 12167 ; http://n2t.net/addgene:12167 ; RRID:Addgene_12167)
For your References section:Defective apoptosis and B-cell lymphomas in mice with p53 point mutation at Ser 23. MacPherson D, Kim J, Kim T, Rhee BK, Van Oostrom CT, DiTullio RA, Venere M, Halazonetis TD, Bronson R, De Vries A, Fleming M, Jacks T. EMBO J. 2004 Sep 15. 23(18):3689-99. 10.1038/sj.emboj.7600363 PubMed 15343266
Map uploaded by the depositor.