Sm-Myocardin Omni-pShuttle
(Plasmid
#122122)
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PurposeExpression of the smooth muscle version of myocardin
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 122122 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepShuttle
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Backbone manufacturerClontech
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMyocardin
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SpeciesM. musculus (mouse)
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Insert Size (bp)2575
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Entrez GeneMyocd (a.k.a. BSAC2A, Srfcp)
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Tag
/ Fusion Protein
- Omni (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Myocardin contains an A435T difference compared to the Myocd NCBI entry. The functional consequence of this difference is unknown.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Sm-Myocardin Omni-pShuttle was a gift from Paul Herring (Addgene plasmid # 122122 ; http://n2t.net/addgene:122122 ; RRID:Addgene_122122) -
For your References section:
The SWI/SNF chromatin remodeling complex regulates myocardin-induced smooth muscle-specific gene expression. Zhou J, Zhang M, Fang H, El-Mounayri O, Rodenberg JM, Imbalzano AN, Herring BP. Arterioscler Thromb Vasc Biol. 2009 Jun;29(6):921-8. doi: 10.1161/ATVBAHA.109.187229. Epub 2009 Apr 2. 10.1161/ATVBAHA.109.187229 PubMed 19342595
Map uploaded by the depositor.
