P22_wtCP_SP141his
(Plasmid #122651)

• Purpose: Expression of P22 virus like particle in bacteria
• Depositing Lab: Trevor Douglas
• Publication: Trevor Douglas lab unpublished plasmids (unpublished)

Backbone

• Vector backbone: pRSF-Duet
• Backbone manufacturer: Novagen
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Use bacterial strain BL21(DE3) for expression.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: P22 Coat: WT / SP: 6xHis-SP141
• Species: bacteriophage P22
• Mutation: truncated SP, P120H in the capsid scaffold (see depositor comments below)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • wtP22-SP141HIS.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Depositor confirms that P120H mutation does not affect plasmid function.