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Top7 V48V
(Plasmid #12393)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 12393 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Plasmids are provided in DH5 alpha, but for protein expression, BL21(DE3)pLysS is recommended.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Top7 V48V
  • Insert Size (bp)
    321
  • Tag / Fusion Protein
    • 6xHis (C terminal on insert)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A synthetic gene with the Top7 sequence under the control of the T7 promoter, a C-terminal 6X His tag, and codon usage optimized for Escherichia coli was obtained from BlueHeron Biotechnologies. The gene was then subcloned into the pet29 b(+) vector from Novagen.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Top7 V48V was a gift from David Baker (Addgene plasmid # 12393 ; http://n2t.net/addgene:12393 ; RRID:Addgene_12393)
  • For your References section:

    Design of a novel globular protein fold with atomic-level accuracy. Kuhlman B, Dantas G, Ireton GC, Varani G, Stoddard BL, Baker D. Science. 2003 Nov 21. 302(5649):1364-8. 10.1126/science.1089427 PubMed 14631033