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(Plasmid #124081)


Item Catalog # Description Quantity Price (USD)
Plasmid 124081 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pEF1 alpha
  • Backbone size w/o insert (bp) 6100
  • Total vector size (bp) 12598
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy


  • Gene/Insert name
    TET1 - full length, mutated
  • Alt name
    Ten Eleven Translocation methylcytosine dioxygenase 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    H1672Y, D1674A
  • Entrez Gene
    TET1 (a.k.a. CXXC6, LCX, bA119F7.1)
  • Promoter EF1 alpha
  • Tags / Fusion Proteins
    • Flag (N terminal on insert)
    • HA (N terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Plasmid expressing wild type full length form of TET1 was taken from addgene (#49792)
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The two mutations listed before (H1672Y, D1674A) were introduced using the
QuickChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF1a_FL MUT TET1 was a gift from Silvia Monticelli (Addgene plasmid # 124081 ; ; RRID:Addgene_124081)
  • For your References section:

    The contribution of active and passive mechanisms of 5mC and 5hmC removal in human T lymphocytes is differentiation- and activation-dependent. Vincenzetti L, Leoni C, Chirichella M, Kwee I, Monticelli S. Eur J Immunol. 2019 Jan 30. doi: 10.1002/eji.201847967. 10.1002/eji.201847967 PubMed 30698829