pEF1a_FL MUT TET1
PurposeExpression of catalytic inactive form of full-length TET1
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||124081||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepEF1 alpha
- Backbone size w/o insert (bp) 6100
- Total vector size (bp) 12598
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Gene/Insert nameTET1 - full length, mutated
Alt nameTen Eleven Translocation methylcytosine dioxygenase 1
SpeciesH. sapiens (human)
Insert Size (bp)6492
Entrez GeneTET1 (a.k.a. CXXC6, LCX, bA119F7.1)
- Promoter EF1 alpha
/ Fusion Proteins
- Flag (N terminal on insert)
- HA (N terminal on insert)
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer TTAGAATTGATCCAAGCTCTCC (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
The two mutations listed before (H1672Y, D1674A) were introduced using the
QuickChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEF1a_FL MUT TET1 was a gift from Silvia Monticelli (Addgene plasmid # 124081 ; http://n2t.net/addgene:124081 ; RRID:Addgene_124081)
For your References section:The contribution of active and passive mechanisms of 5mC and 5hmC removal in human T lymphocytes is differentiation- and activation-dependent. Vincenzetti L, Leoni C, Chirichella M, Kwee I, Monticelli S. Eur J Immunol. 2019 Jan 30. doi: 10.1002/eji.201847967. 10.1002/eji.201847967 PubMed 30698829