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FUT8-Dox
(Plasmid #124631)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 124631 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 124631-D50 50 μg of DNA in Tris buffer $495
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DNA 124631-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pJAZZ
  • Backbone manufacturer
    Lucigen
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin, 100 & 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    Alpha-(1,6)-fucosyltransferase
  • Alt name
    FUT8
  • Species
    Cricetulus griseus
  • Insert Size (bp)
    1728
  • Promoter TRE-tight

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GGTTTACCGAGCTCTTATTGGTTTTCAAACTTCATTG
  • 3′ sequencing primer GGATCTTAAAAACATTATACAATACTACAAGCATAAAAACGCAC
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Tetracycline repressor A3 mutant
  • Alt name
    rtTA3
  • Insert Size (bp)
    708
  • Promoter hEF1a

Cloning Information for Gene/Insert 2

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GGTGCGTTTTTATGCTTGTAGTATTGTATAATGTTTTTAAGATC
  • 3′ sequencing primer GGCGTATAAAACATCTGGATAAGACGAGAGATTGG
  • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    TagFP635
  • Alt name
    mKate
  • Insert Size (bp)
    713
  • Promoter hEF1a

Cloning Information for Gene/Insert 3

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CCAATCTCTCGTCTTATCCAGATGTTTTATACGCC
  • 3′ sequencing primer CTGCCTTTTACACTCACATAAGGGAATTCACC
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FUT8-Dox was a gift from Ron Weiss (Addgene plasmid # 124631 ; http://n2t.net/addgene:124631 ; RRID:Addgene_124631)

  • For your References section:

    Small-molecule control of antibody N-glycosylation in engineered mammalian cells. Chang MM, Gaidukov L, Jung G, Tseng WA, Scarcelli JJ, Cornell R, Marshall JK, Lyles JL, Sakorafas P, Chu AA, Cote K, Tzvetkova B, Dolatshahi S, Sumit M, Mulukutla BC, Lauffenburger DA, Figueroa B Jr, Summers NM, Lu TK, Weiss R. Nat Chem Biol. 2019 May 20. pii: 10.1038/s41589-019-0288-4. doi: 10.1038/s41589-019-0288-4. 10.1038/s41589-019-0288-4 PubMed 31110306
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