pCAGGS-mTagBFP2-T2A-dre
(Plasmid
#124832)
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PurposeMonomeric TagBFP2 and dre recombinase
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Depositing Lab
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Depositing OrganizationUniversity of Utah
Located in United States of America -
Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 124832 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 124832-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 124832-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCAGGS
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH10B
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemTagBFP2-T2A-dre
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SpeciesSynthetic
- Promoter CAG
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pCAG-F
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bymTagBFP2 is from Michael Davidson. dre is from Francis Stewart.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
In the NGS sequence, polymorphisms are present in a GC-rich region of the Chicken beta-actin promoter that do not affect the function of the plasmid.
DNA (Catalog # 124832-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 124832-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAGGS-mTagBFP2-T2A-dre was a gift from Mario Capecchi (Addgene plasmid # 124832 ; http://n2t.net/addgene:124832 ; RRID:Addgene_124832)
