Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12507||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4700
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneRUNX1T1 (a.k.a. AML1-MTG8, AML1T1, CBFA2T1, CDR, ETO, MTG8, ZMYND2)
/ Fusion Protein
- 3xFlag (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (blunted) (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer pCEP fwd (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCMV-3xFlag-ETO was a gift from Dong-Er Zhang (Addgene plasmid # 12507 ; http://n2t.net/addgene:12507 ; RRID:Addgene_12507)
For your References section:AML1/RUNX1 phosphorylation by cyclin-dependent kinases regulates the degradation of AML1/RUNX1 by the anaphase-promoting complex. Biggs JR, Peterson LF, Zhang Y, Kraft AS, Zhang DE. Mol Cell Biol. 2006 Oct . 26(20):7420-9. 10.1128/MCB.00597-06 PubMed 17015473