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pCAGGS-Akt-FoxO3a-KTR-EGFP
(Plasmid #125131)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 125131 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 125131-D50 50 μg of DNA in Tris buffer $495
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DNA 125131-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pCAGGS
  • Backbone manufacturer
    Junichi Miyazaki (Osaka University, Japan)
  • Backbone size w/o insert (bp) 4870
  • Total vector size (bp) 8551
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FOXO2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1206
  • Mutation
    FoxO3A 1-402 amino acid
  • GenBank ID
    NM_001455
  • Entrez Gene
    FOXO3 (a.k.a. AF6q21, FKHRL1, FKHRL1P2, FOXO2, FOXO3A)
  • Promoter CAG promoter
  • Tag / Fusion Protein
    • Enhanced green fluorescent protein (EGFP) (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer pCAGGSseq_F
  • 3′ sequencing primer pCAGGSseq_R
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Expression vector for Akt-FoxO3a-KTR-EGFP in mammalian cells.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAGGS-Akt-FoxO3a-KTR-EGFP was a gift from Kazuhiro Aoki (Addgene plasmid # 125131 ; http://n2t.net/addgene:125131 ; RRID:Addgene_125131)

  • For your References section:

    Multiplexed Fluorescence Imaging of ERK and Akt Activities and Cell-cycle Progression. Maryu G, Matsuda M, Aoki K. Cell Struct Funct. 2016 Jul 22;41(2):81-92. doi: 10.1247/csf.16007. Epub 2016 May 28. 10.1247/csf.16007 PubMed 27247077
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