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pT3TS-HiFi-nCas9n-A72
(Plasmid #125646)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 125646 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 125646-D50 50 μg of DNA in Tris buffer $495
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DNA 125646-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pT3TS
  • Backbone size w/o insert (bp) 3310
  • Total vector size (bp) 7490
  • Modifications to backbone
    polyA insert (72 As with restriction sites) was inserted
  • Vector type
    RNA in vitro transcription vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HiFi-nCas9n
  • Alt name
    HiFi-Cas9
  • Species
    Streptococcus pyogenes
  • Insert Size (bp)
    4179
  • Mutation
    R691A from wild-type Cas9
  • Promoter T3

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Originally obtained from https://www.addgene.org/46757/, polyA insert was added and the HiFi R691A mutation was introduced.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT3TS-HiFi-nCas9n-A72 was a gift from Jason Berman (Addgene plasmid # 125646 ; http://n2t.net/addgene:125646 ; RRID:Addgene_125646)

  • For your References section:

    Genome Editing in Zebrafish Using High-Fidelity Cas9 Nucleases: Choosing the Right Nuclease for the Task. Prykhozhij SV, Cordeiro-Santanach A, Caceres L, Berman JN. Methods Mol Biol. 2020;2115:385-405. doi: 10.1007/978-1-0716-0290-4_21. 10.1007/978-1-0716-0290-4_21 PubMed 32006412
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