|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12636||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6600
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)1400
Entrez GeneSMAD2 (a.k.a. JV18, JV18-1, MADH2, MADR2, hMAD-2, hSMAD2)
/ Fusion Protein
- Flag (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site HpaI (destroyed during cloning)
- 3′ cloning site HpaI (destroyed during cloning)
- 5′ sequencing primer LNCX (Common Sequencing Primers)
LPCX was made by replacing the neo gene of LNCX with the puro gene.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:LPCX Smad2 was a gift from Rik Derynck (Addgene plasmid # 12636 ; http://n2t.net/addgene:12636 ; RRID:Addgene_12636)
For your References section:Roles of autocrine TGF-beta receptor and Smad signaling in adipocyte differentiation. Choy L, Skillington J, Derynck R. J Cell Biol. 2000 May 1. 149(3):667-82. 10.1083/jcb.149.3.667 PubMed 10791980