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PurposeDoxycycline inducible expression of UPRT and CD along with reporter RFP
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Depositing Lab
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Depositing OrganizationMemorial Sloan-Kettering Cancer Center
Located in the United States of America -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 126677 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 126677-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 126677-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepLenti CMVtight Puro
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Backbone manufactureraddgene
- Backbone size w/o insert (bp) 7900
- Total vector size (bp) 9900
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Modifications to backbonecloned out eGFP
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameUPRT-T2A-RFP-IRES-CD
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Alt nameuracil phosphoribosyltransferase
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Alt nameTGME49_312480
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Alt namecytosine-deaminase
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SpeciesS. cerevisiae (budding yeast); Toxoplasma gondii
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Insert Size (bp)2000
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GenBank IDXP_002364470.1
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Entrez GeneFCY1 (a.k.a. YPR062W)
- Promoter tight TRE
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMVtightFORW
- 3′ sequencing primer WPRErev
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byUPRT: Addgene #47110; CD: Addgene #35102; pLenti CMVtight vector: Addgene #26431
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 126677-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 126677-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMVtight-UPRT-T2A-RFP-IRES-CD was a gift from Joan Massague (Addgene plasmid # 126677 ; http://n2t.net/addgene:126677 ; RRID:Addgene_126677) -
For your References section:
Flura-seq identifies organ-specific metabolic adaptations during early metastatic colonization. Basnet H, Tian L, Ganesh K, Huang YH, Macalinao DG, Brogi E, Finley LW, Massague J. Elife. 2019 Mar 26;8. pii: 43627. doi: 10.7554/eLife.43627. 10.7554/eLife.43627 PubMed 30912515
Map uploaded by the depositor.
