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pAAV-CAG-DIO-tTA
(Plasmid #127091)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 127091 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    tTA
  • Insert Size (bp)
    747
  • Mutation
    *(see below)
  • Promoter CAG

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AscI (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

*Note: This construct is based on Addgene ID 99121 (the ihSyn1 promoter was changed to the CAG promoter). The tTA was made from a reverse tetracycline transactivator (rtTA) by changing two amino acids (G19E and P56A). These two residue changes have been shown to be sufficient for the reverse phenotype (10.1073/pnas.130192197).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-CAG-DIO-tTA was a gift from Viviana Gradinaru (Addgene plasmid # 127091 ; http://n2t.net/addgene:127091 ; RRID:Addgene_127091)
  • For your References section:

    Identification of peripheral neural circuits that regulate heart rate using optogenetic and viral vector strategies. Rajendran PS, Challis RC, Fowlkes CC, Hanna P, Tompkins JD, Jordan MC, Hiyari S, Gabris-Weber BA, Greenbaum A, Chan KY, Deverman BE, Munzberg H, Ardell JL, Salama G, Gradinaru V, Shivkumar K. Nat Commun. 2019 Apr 26;10(1):1944. doi: 10.1038/s41467-019-09770-1. 10.1038/s41467-019-09770-1 PubMed 31028266