pAAV-CAG-DIO-tTA
(Plasmid #127091)

• Purpose: An AAV genome with Cre-dependent expression of tTA from the CAG promoter
• Depositing Lab: Viviana Gradinaru
• Publication: Rajendran et al Nat Commun. 2019 Apr 26;10(1):1944. doi: 10.1038/s41467-019-09770-1.

Backbone

• Vector backbone: pAAV
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: tTA
• Insert Size (bp): 747
• Mutation: *(see below)
• Promoter: CAG

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AscI (not destroyed)
• 3′ cloning site: NheI (not destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • pAAV-CAG-DIO-tTA.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

*Note: This construct is based on Addgene ID 99121 (the ihSyn1 promoter was changed to the CAG promoter). The tTA was made from a reverse tetracycline transactivator (rtTA) by changing two amino acids (G19E and P56A). These two residue changes have been shown to be sufficient for the reverse phenotype (10.1073/pnas.130192197).