PurposeAn AAV genome with Cre-dependent expression of tTA from the CAG promoter
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||127091||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, AAV
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Insert Size (bp)747
- Promoter CAG
- Cloning method Restriction Enzyme
- 5′ cloning site AscI (not destroyed)
- 3′ cloning site NheI (not destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
*Note: This construct is based on Addgene ID 99121 (the ihSyn1 promoter was changed to the CAG promoter). The tTA was made from a reverse tetracycline transactivator (rtTA) by changing two amino acids (G19E and P56A). These two residue changes have been shown to be sufficient for the reverse phenotype (10.1073/pnas.130192197).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAAV-CAG-DIO-tTA was a gift from Viviana Gradinaru (Addgene plasmid # 127091 ; http://n2t.net/addgene:127091 ; RRID:Addgene_127091)
For your References section:Identification of peripheral neural circuits that regulate heart rate using optogenetic and viral vector strategies. Rajendran PS, Challis RC, Fowlkes CC, Hanna P, Tompkins JD, Jordan MC, Hiyari S, Gabris-Weber BA, Greenbaum A, Chan KY, Deverman BE, Munzberg H, Ardell JL, Salama G, Gradinaru V, Shivkumar K. Nat Commun. 2019 Apr 26;10(1):1944. doi: 10.1038/s41467-019-09770-1. 10.1038/s41467-019-09770-1 PubMed 31028266