41 pBS119 d10L
(Plasmid #13065)

• Depositing Lab: Nathan Lawson
• Publication: Lawson et al Development. 2001 Oct . 128(19):3675-83.

Backbone

• Vector backbone: na
• Backbone size w/o insert (bp): 2950

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: fli genomic
• Species: D. rerio (zebrafish)
• Insert Size (bp): 7017
• Entrez Gene: fli1 (a.k.a. cb855, fli, fli-1, fli1a, wu:fc45b11)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T3
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

An XbaI fragment of the zebrafish fli1 promoter that includes 1 kb upstream of exon 1 and 6 kb of intron 1 was subcloned into pBluescript from a PAC obtained by hybridization to the 5' end of the fli1 cDNA. An oligonucleotide linker containing NheI, SrfI, and AscI sites was cloned into an MluI site upstream of the fli1 start codon to give pBS119d10L.