AAVS1 KanR foundation lox casette
(Plasmid #132388)

• Purpose: Foundation cassette containing lox sites for cre-mediated exchange of inducible vectors, confers mammalian G418 resistance, targets AAVS1 locus
• Depositing Lab: Gerold Schmitt-Ulms
• Publication: Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi: 10.21769/BioProtoc.3615. eCollection 2020 May 5.

Backbone

• Vector backbone: AAVS1-T2A-Puro-Cas9-TREtight
• Backbone manufacturer: Addgene
• Total vector size (bp): 6226
• Vector type: Mammalian Expression, Cre/Lox, CRISPR
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: G418 resistance
• Alt name: KanR
• Alt name: NeoR
• GenBank ID: X01702
• Promoter: promoterless

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AsiSI (not destroyed)
• 3′ cloning site: BbvCI (not destroyed)
• 5′ sequencing primer: TCATGGCATCTTCCAGGGG

Resource Information

• Supplemental Documents:
  • AAVS1-lox71-Frt-KanR-Frt-lox2272.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note: Plasmid contains a D238Y mutation in NeoR/KanR, which is not known to affect plasmid function.