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pCMV-T7-AcrIIA4-NLS(SV40) (KAC200)
(Plasmid #133801)


Item Catalog # Description Quantity Price (USD)
Plasmid 133801 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    JDS246 (Plasmid #43861)
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    human codon optimized AcrIIA4
  • Alt name
  • Species
  • Mutation
  • Promoter CMV and T7
  • Tag / Fusion Protein
    • NLS(SV40) (C terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer oBK6928-CCAAGTCTCCACCCCATTGACG
  • 3′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-T7-AcrIIA4-NLS(SV40) (KAC200) was a gift from Joseph Bondy-Denomy & Benjamin Kleinstiver (Addgene plasmid # 133801 ; ; RRID:Addgene_133801)
  • For your References section:

    Broad-spectrum anti-CRISPR proteins facilitate horizontal gene transfer. Mahendra C, Christie KA, Osuna BA, Pinilla-Redondo R, Kleinstiver BP, Bondy-Denomy J. Nat Microbiol. 2020 Apr;5(4):620-629. doi: 10.1038/s41564-020-0692-2. Epub 2020 Mar 26. 10.1038/s41564-020-0692-2 PubMed 32218510