Skip to main content
Addgene

pGEX4T1-Smurf1 C699A
(Plasmid #13503)

Full plasmid sequence is not available for this item.

Loading...

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 13503 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

* Log in to view industry pricing.

Backbone

  • Vector backbone
    pGEX-4T1
  • Backbone size w/o insert (bp) 4900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SMURF1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2100
  • Mutation
    C699A: catalytically inactive
  • GenBank ID
    NM_020429
  • Entrez Gene
    SMURF1
  • Tag / Fusion Protein
    • GST (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer pGEX 5'
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Vector has TEV site.

A human Smurf1 cDNA encoding all but the first eight amino acids was identified in the expressed-sequence-tag database (AA292123), and full-length hSmurf1 was reconstituted using a fragment of XSmurf1 cDNA encoding the first eight amino acids.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX4T1-Smurf1 C699A was a gift from Jeff Wrana (Addgene plasmid # 13503 ; http://n2t.net/addgene:13503 ; RRID:Addgene_13503)
  • For your References section:

    A SMAD ubiquitin ligase targets the BMP pathway and affects embryonic pattern formation. Zhu H, Kavsak P, Abdollah S, Wrana JL, Thomsen GH. Nature. 1999 Aug 12. 400(6745):687-93. 10.1038/23293 PubMed 10458166