PurposeEncodes Marburg virus VP35 gene under control of the AG promoter
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||135471||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
Currently unavailable outside the U.S.
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesMarburg marburgvirus, Musoke variant
- Promoter AG
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI/StuI (not destroyed)
- 3′ cloning site EcoRI/SmaI (destroyed during cloning)
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
VP35 gene flanked by BamHI sites
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCAGGS_VP35_MARV was a gift from Elke Mühlberger (Addgene plasmid # 135471 ; http://n2t.net/addgene:135471 ; RRID:Addgene_135471)
For your References section:Recombinant Marburg virus expressing EGFP allows rapid screening of virus growth and real-time visualization of virus spread. Schmidt KM, Schumann M, Olejnik J, Krahling V, Muhlberger E. J Infect Dis. 2011 Nov;204 Suppl 3:S861-70. doi: 10.1093/infdis/jir308. 10.1093/infdis/jir308 PubMed 21987762