PurposeMammalian expression vector encoding Marburg virus VP30 gene under control of the AG promoter
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||135472||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
Currently unavailable outside the U.S.
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesMarburg marburgvirus, Musoke variant
- Promoter AG
- Cloning method Restriction Enzyme
- 5′ cloning site SmaI/StuI (destroyed during cloning)
- 3′ cloning site SmaI (destroyed during cloning)
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
VP30 gene flanked by BamHI sites
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCAGGS_VP30_MARV was a gift from Elke Mühlberger (Addgene plasmid # 135472 ; http://n2t.net/addgene:135472 ; RRID:Addgene_135472)
For your References section:A Chimeric Lloviu Virus Minigenome System Reveals that the Bat-Derived Filovirus Replicates More Similarly to Ebolaviruses than Marburgviruses. Manhart WA, Pacheco JR, Hume AJ, Cressey TN, Deflube LR, Muhlberger E. Cell Rep. 2018 Sep 4;24(10):2573-2580.e4. doi: 10.1016/j.celrep.2018.08.008. 10.1016/j.celrep.2018.08.008 PubMed 30184492