PurposeEncodes Marburg virus minigenome with eGFP reporter gene under control of a T7 RNA polymerase promoter
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||135474||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
Currently unavailable outside the U.S.
This material is available to academics and nonprofits only.
Backbone manufacturerAndrew Ball, University of Alabama at Birmingham
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameMarburg virus minigenome, eGFP reporter
SpeciesMarburg marburgvirus, Musoke variant
- Promoter T7
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p2,0_3M5M_eGFP was a gift from Elke Mühlberger (Addgene plasmid # 135474 ; http://n2t.net/addgene:135474 ; RRID:Addgene_135474)
For your References section:A Chimeric Lloviu Virus Minigenome System Reveals that the Bat-Derived Filovirus Replicates More Similarly to Ebolaviruses than Marburgviruses. Manhart WA, Pacheco JR, Hume AJ, Cressey TN, Deflube LR, Muhlberger E. Cell Rep. 2018 Sep 4;24(10):2573-2580.e4. doi: 10.1016/j.celrep.2018.08.008. 10.1016/j.celrep.2018.08.008 PubMed 30184492