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(Plasmid #135621)


Item Catalog # Description Quantity Price (USD)
Plasmid 135621 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 4093
  • Total vector size (bp) 5040
  • Modifications to backbone
    ss-bfe1 removed and replaced with ss-bfe3
  • Vector type
    Bacteroides suicide vector with inducible counter-selection toxin

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
    S17 lambda pir
  • Copy number


  • Gene/Insert name
  • Alt name
  • Species
    Bacteroides fragilis
  • Insert Size (bp)
  • GenBank ID
  • Promoter Bacteroides promoter regualted by TetR transcriptional regulator
  • Tag / Fusion Protein
    • signal sequence for periplasmic targeting (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer acatataaaagaaaagacacatgaagaaaattttatctttgc
  • 3′ sequencing primer ctgcagcccgggggatccactcatctgatactatgccaatg
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLGB36 was a gift from Laurie Comstock (Addgene plasmid # 135621 ; ; RRID:Addgene_135621)
  • For your References section:

    Genetic and Biochemical Analysis of Anaerobic Respiration in Bacteroides fragilis and Its Importance In Vivo. Ito T, Gallegos R, Matano LM, Butler NL, Hantman N, Kaili M, Coyne MJ, Comstock LE, Malamy MH, Barquera B. mBio. 2020 Feb 4;11(1). pii: mBio.03238-19. doi: 10.1128/mBio.03238-19. 10.1128/mBio.03238-19 PubMed 32019804