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(Plasmid #13574)


Item Catalog # Description Quantity Price (USD)
Plasmid 13574 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4400
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    human peroxisome proliferator activated receptor alpha A/B domain deletion construct (aa101-468)
  • Alt name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    deleted residues 1-100.
  • GenBank ID
  • Entrez Gene
    PPARA (a.k.a. NR1C1, PPAR, PPARalpha, hPPAR)
  • Tag / Fusion Protein
    • x6His N-terminal, enterokinase cleavage site

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer F1 CCAGAACAAAGAAGCTG from 495 to 500; F2 GACCTGAAATCTCTGGC
  • 3′ sequencing primer CAGCAGGGTAACCTGGTC from 616 to 633
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

This plasmid contains an unidentified ~2.5kb band when run on an agarose gel. It is unknown whether this extra band affects plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTrcHisA-PPARalpha(aa101-468) was a gift from Martin Scanlon (Addgene plasmid # 13574 ; ; RRID:Addgene_13574)