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pHAGE2-TetOminiCMV-OSM
(Plasmid #136555)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 136555 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pHAGE2
  • Backbone size w/o insert (bp) 6000
  • Total vector size (bp) 9736
  • Vector type
    Bacterial Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Oct4-P2A-Sox2-E2A-cMyc
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3500
  • Entrez Gene
    Myc (a.k.a. AU016757, Myc2, N, Niard, Nird, bHLHe3, bHLHe39)
  • Entrez Gene
    Pou5f1 (a.k.a. NF-A3, Oct, Oct-, Oct-3, Oct-3/, Oct-3/4, Oct-4, Oct3, Oct3/, Oct3/4, Oct4, Otf, Otf-, Otf-3, Otf-4, Otf3, Otf3-, Otf3-rs7, Otf3g, Otf4)
  • Entrez Gene
    Sox2 (a.k.a. Sox, Sox-2, lc, lcc, ys, ysb)
  • Promoter tetO-miniCMV (dox-inducible)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site ClaI (not destroyed)
  • 5′ sequencing primer GAGACGCCATCCACGCTGT
  • 3′ sequencing primer AGGAAGGTCCGCTGGATTGA
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHAGE2-TetOminiCMV-OSM was a gift from Hans Schöler (Addgene plasmid # 136555 ; http://n2t.net/addgene:136555 ; RRID:Addgene_136555)
  • For your References section:

    Excluding Oct4 from Yamanaka Cocktail Unleashes the Developmental Potential of iPSCs. Velychko S, Adachi K, Kim KP, Hou Y, MacCarthy CM, Wu G, Scholer HR. Cell Stem Cell. 2019 Oct 30. pii: S1934-5909(19)30423-0. doi: 10.1016/j.stem.2019.10.002. 10.1016/j.stem.2019.10.002 PubMed 31708402