cmv 16 E7
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13686||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepCMV bam neo
- Backbone size w/o insert (bp) 6550
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameHPV 16 E7
SpeciesH. sapiens (human)
Insert Size (bp)300
Entrez GeneE7 (a.k.a. HpV16gp2)
- Cloning method Restriction Enzyme
- 5′ cloning site BamH1 (not destroyed)
- 3′ cloning site BamH1 (not destroyed)
- 5′ sequencing primer B glob intron-F
- 3′ sequencing primer B glob-pA-R (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:cmv 16 E7 was a gift from Karl Munger (Addgene plasmid # 13686 ; http://n2t.net/addgene:13686 ; RRID:Addgene_13686)
For your References section:Degradation of the retinoblastoma tumor suppressor by the human papillomavirus type 16 E7 oncoprotein is important for functional inactivation and is separable from proteasomal degradation of E7. Gonzalez SL, Stremlau M, He X, Basile JR, Munger K. J Virol. 2001 Aug . 75(16):7583-91. 10.1128/JVI.75.16.7583-7591.2001 PubMed 11462030