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pTetON-BMP2/7
(Plasmid #137913)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 137913 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pTREtight-BI
  • Backbone manufacturer
    Takara/Clontech
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    BMP2
  • Species
    H. sapiens (human)
  • Entrez Gene
    BMP2 (a.k.a. BDA2, BMP2A, SSFSC, SSFSC1)
  • Promoter TRE

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer Unknown
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    BMP7
  • Species
    H. sapiens (human)
  • GenBank ID
  • Entrez Gene
    BMP7 (a.k.a. OP-1)
  • Promoter TRE

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer Unknown
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTetON-BMP2/7 was a gift from Georg Feichtinger (Addgene plasmid # 137913 ; http://n2t.net/addgene:137913 ; RRID:Addgene_137913)
  • For your References section:

    Constitutive and inducible co-expression systems for non-viral osteoinductive gene therapy. Feichtinger GA, Hacobian A, Hofmann AT, Wassermann K, Zimmermann A, van Griensven M, Redl H. Eur Cell Mater. 2014 Feb 19;27:166-84; discussion 184. PubMed 24554273