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(Plasmid #138201)


Item Catalog # Description Quantity Price (USD)
Plasmid 138201 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 3000
  • Total vector size (bp) 4000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    E Coli tRNA Guanine Transglycosylase-His
  • Alt name
  • Species
    E. Coli
  • Insert Size (bp)
  • Promoter T7 promoter
  • Tag / Fusion Protein
    • His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PstI (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer T7 Promoter
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Seth Alexander

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

J. Am. Chem. Soc. 2015, 137, 40, 12756-12759

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TGT-His was a gift from Neal Devaraj (Addgene plasmid # 138201 ; ; RRID:Addgene_138201)
  • For your References section:

    Enzymatic covalent labeling of RNA with RNA transglycosylation at guanosine (RNA-TAG). Busby KN, Devaraj NK. Methods Enzymol. 2020;641:373-399. doi: 10.1016/bs.mie.2020.03.009. Epub 2020 Apr 20. 10.1016/bs.mie.2020.03.009 PubMed 32713531