pcDNA-c/o NL4-3 Env delta CT
(Plasmid #139285)

• Purpose: HIV-1 NL4-3 glycoprotein lacking cytoplasmic tail
• Depositing Lab: Jeremy Luban
• Publication: Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051. doi: 10.1038/s41564-019-0592-5. Epub 2019 Oct 21.

Backbone

• Vector backbone: pcDNA3.1 (-)
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: codon optimized HIV-1 Env delta cytoplasmic tail
• Species: Synthetic
• Insert Size (bp): 2133
• Mutation: deletion of cytoplasmic tail after residue 710
• GenBank ID: AAA44992.2
• Promoter: CMV IE

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: BGH reverse

Resource Information

• Supplemental Documents:
  • pcDNA-c_o NL4-3 Env delta CT.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry