pAAV-CAG-DIO-PPO-Venus
(Plasmid #139504)

• Purpose: AAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the CAG promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades.
• Depositing Lab: Michael Bruchas
• Publication: Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11. doi: 10.1016/j.neuron.2021.04.026. Epub 2021 May 11.

Backbone

• Vector backbone: pAAV-CAG-DIO-eYFP
  (Search Vector Database)
• Backbone manufacturer: Zweiffel lab
• Backbone size w/o insert (bp): 4000
• Total vector size (bp): 6000
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: parapinopsin
• Alt name: PPO
• Species: Lethenteron camtschaticum
• Insert Size (bp): 1029
• GenBank ID: AB116380.1
• Promoter: CAG
• Tag / Fusion Protein:
  • Venus tag following 3x Ala linker (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (unknown if destroyed)
• 3′ cloning site: AscI (unknown if destroyed)
• 5′ sequencing primer: CAG
• 3′ sequencing primer: WPRE

Resource Information

• Supplemental Documents:
  • pAAV-CAG-DIO-PPO-Venus
• A portion of this plasmid was derived from a plasmid made by: parapinopsin was originally cloned by Koyanagi et al., PNAS 2004

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2021.02.19.432008v1 for bioRxiv preprint.