Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pAAV-CAG-DIO-PPO-Venus
(Plasmid #139504)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 139504 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAAV-CAG-DIO-eYFP
  • Backbone manufacturer
    Zweiffel lab
  • Backbone size w/o insert (bp) 4000
  • Total vector size (bp) 6000
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    parapinopsin
  • Alt name
    PPO
  • Species
    Lethenteron camtschaticum
  • Insert Size (bp)
    1029
  • GenBank ID
    AB116380.1
  • Promoter CAG
  • Tag / Fusion Protein
    • Venus tag following 3x Ala linker (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (unknown if destroyed)
  • 3′ cloning site AscI (unknown if destroyed)
  • 5′ sequencing primer CAG
  • 3′ sequencing primer WPRE
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    parapinopsin was originally cloned by Koyanagi et al., PNAS 2004
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-CAG-DIO-PPO-Venus was a gift from Michael Bruchas (Addgene plasmid # 139504 ; http://n2t.net/addgene:139504 ; RRID:Addgene_139504)
  • For your References section:

    A photoswitchable GPCR-based opsin for presynaptic silencing. Copits BA, O’Neill PR, Gowrishankar R, Yoo JJ, Meshik X, Parker KE, Spangler SM, Vasquez AM, Elerding AJ, Stander MC, Kalyanaraman V, Vogt SK, Samineni VK, Gautam N, Sunahara RK, Gereau RW, Bruchas MR.. BioRxiv 10.1101/2021.02.19.432008