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pAAV-Ef1a-DIO-PPO-Venus
(Plasmid #139505)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 139505 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAAV-Ef1a-DIO-eYFP
  • Backbone manufacturer
    Deisseroth lab
  • Backbone size w/o insert (bp) 5681
  • Total vector size (bp) 7439
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    parapinopsin
  • Alt name
    PPO
  • Species
    Lethenteron camtschaticum
  • Insert Size (bp)
    1029
  • GenBank ID
    AB116380.1
  • Promoter Ef1a
  • Tag / Fusion Protein
    • Venus tag following 3x Ala linker (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AscI (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer T7 forward primer
  • 3′ sequencing primer 5’-GGCACAGTCGAGGCGCGCCAGCGGGTTTAAACG-3’
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

See Koyanagi et al., PNAS 2004 (PMID: 15096614) for original cloning of lamprey parapinopsin. Please visit https://www.biorxiv.org/content/10.1101/2021.02.19.432008v1 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-Ef1a-DIO-PPO-Venus was a gift from Michael Bruchas (Addgene plasmid # 139505 ; http://n2t.net/addgene:139505 ; RRID:Addgene_139505)
  • For your References section:

    A photoswitchable GPCR-based opsin for presynaptic inhibition. Copits BA, Gowrishankar R, O'Neill PR, Li JN, Girven KS, Yoo JJ, Meshik X, Parker KE, Spangler SM, Elerding AJ, Brown BJ, Shirley SE, Ma KKL, Vasquez AM, Stander MC, Kalyanaraman V, Vogt SK, Samineni VK, Patriarchi T, Tian L, Gautam N, Sunahara RK, Gereau RW 4th, Bruchas MR. Neuron. 2021 Jun 2;109(11):1791-1809.e11. doi: 10.1016/j.neuron.2021.04.026. Epub 2021 May 11. 10.1016/j.neuron.2021.04.026 PubMed 33979635