|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13988||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4900
Vector typeBacterial Expression
Growth in Bacteria
Copy numberLow Copy
SpeciesM. musculus (mouse)
Insert Size (bp)920
Entrez GeneNanog (a.k.a. 2410002E02Rik, ENK, ecat4)
/ Fusion Protein
- His (N terminal on backbone)
- Cloning method Gateway Cloning
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pDEST17-Nanog was a gift from Shinya Yamanaka (Addgene plasmid # 13988 ; http://n2t.net/addgene:13988 ; RRID:Addgene_13988)
For your References section:The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells. Mitsui K, Tokuzawa Y, Itoh H, Segawa K, Murakami M, Takahashi K, Maruyama M, Maeda M, Yamanaka S. Cell. 2003 May 30. 113(5):631-42. 10.1016/S0092-8674(03)00393-3 PubMed 12787504
Map uploaded by the depositor.