|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13459||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6800
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Insert Size (bp)950
Entrez GeneSox2 (a.k.a. Sox-2, lcc, ysb)
/ Fusion Protein
- HA (N terminal on backbone)
- Cloning method Gateway Cloning
- 5′ sequencing primer pCAG-F (5'-GCAACGTGCTGGTTATTGTG-3')
- 3′ sequencing primer IRES-R (5'-GCATTCCTTTGGCGAGAG-3') (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byCAG promoter was from Dr. Jun-ichi Miyazaki of Osaka University Graduate School of Medicine. In publication using this plasmid, please cite: Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108:193-200, 1991. Niwa, H., Yamamura, K. & Miyazaki, J.
Articles Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
The Sox2 cDNA contains a modified ATG start codon to TTG for cloning into vectors with an N-terminal tag.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCAG-HA-Sox2-IP was a gift from Shinya Yamanaka (Addgene plasmid # 13459 ; http://n2t.net/addgene:13459 ; RRID:Addgene_13459)
For your References section:Differential roles for Sox15 and Sox2 in transcriptional control in mouse embryonic stem cells. Maruyama M, Ichisaka T, Nakagawa M, Yamanaka S. J Biol Chem. 2005 Jul 1. 280(26):24371-9. 10.1074/jbc.M501423200 PubMed 15863505