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pCAG-CBE4max-SpRY-P2A-EGFP (RTW5133) Citations (12)

Originally described in: Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants.
Walton RT, Christie KA, Whittaker MN, Kleinstiver BP Science. 2020 Apr 17;368(6488):290-296. doi: 10.1126/science.aba8853. Epub 2020 Mar 26. PubMed Journal

Articles Citing pCAG-CBE4max-SpRY-P2A-EGFP (RTW5133)

Articles
Small-molecule compounds boost genome-editing efficiency of cytosine base editor. Zhao T, Li Q, Zhou C, Lv X, Liu H, Tu T, Tang N, Cheng Y, Liu X, Liu C, Zhao J, Song Z, Wang H, Li J, Gu F. Nucleic Acids Res. 2021 Sep 7;49(15):8974-8986. doi: 10.1093/nar/gkab645. PubMed
Saturation variant interpretation using CRISPR prime editing. Erwood S, Bily TMI, Lequyer J, Yan J, Gulati N, Brewer RA, Zhou L, Pelletier L, Ivakine EA, Cohn RD. Nat Biotechnol. 2022 Jun;40(6):885-895. doi: 10.1038/s41587-021-01201-1. Epub 2022 Feb 21. PubMed
Multiplex base- and prime-editing with drive-and-process CRISPR arrays. Yuan Q, Gao X. Nat Commun. 2022 May 19;13(1):2771. doi: 10.1038/s41467-022-30514-1. PubMed

Associated Plasmids
Disease modeling by efficient genome editing using a near PAM-less base editor in vivo. Rosello M, Serafini M, Mignani L, Finazzi D, Giovannangeli C, Mione MC, Concordet JP, Del Bene F. Nat Commun. 2022 Jun 15;13(1):3435. doi: 10.1038/s41467-022-31172-z. PubMed
PAM-flexible dual base editor-mediated random mutagenesis and self-activation strategies to improve CRISPRa potency. Lau CH, Huang S, Lam RHW, Tin C. Mol Ther Methods Clin Dev. 2022 May 29;26:26-37. doi: 10.1016/j.omtm.2022.05.005. eCollection 2022 Sep 8. PubMed
Transcription factor antagonism regulates heterogeneity in embryonic stem cell states. Hu S, Metcalf E, Mahat DB, Chan L, Sohal N, Chakraborty M, Hamilton M, Singh A, Singh A, Lees JA, Sharp PA, Garg S. Mol Cell. 2022 Dec 1;82(23):4410-4427.e12. doi: 10.1016/j.molcel.2022.10.022. Epub 2022 Nov 9. PubMed
LMNA Co-Regulated Gene Expression as a Suitable Readout after Precise Gene Correction. Wang H, Krause A, Escobar H, Muthel S, Metzler E, Spuler S. Int J Mol Sci. 2022 Dec 8;23(24):15525. doi: 10.3390/ijms232415525. PubMed
PAM-flexible genome editing with an engineered chimeric Cas9. Zhao L, Koseki SRT, Silverstein RA, Amrani N, Peng C, Kramme C, Savic N, Pacesa M, Rodriguez TC, Stan T, Tysinger E, Hong L, Yudistyra V, Ponnapati MR, Jacobson JM, Church GM, Jakimo N, Truant R, Jinek M, Kleinstiver BP, Sontheimer EJ, Chatterjee P. Nat Commun. 2023 Oct 4;14(1):6175. doi: 10.1038/s41467-023-41829-y. PubMed

Associated Plasmids
One-pot DTECT enables rapid and efficient capture of genetic signatures for precision genome editing and clinical diagnostics. Baudrier L, Benamozig O, Langley J, Chopra S, Kalashnikova T, Benaoudia S, Singh G, Mahoney DJ, Wright NAM, Billon P. Cell Rep Methods. 2024 Feb 26;4(2):100698. doi: 10.1016/j.crmeth.2024.100698. Epub 2024 Jan 31. PubMed
CRISPR Base Editing to Create Potential Charcot-Marie-Tooth Disease Models with High Editing Efficiency: Human Induced Pluripotent Stem Cell Harboring SH3TC2 Variants. Loret C, Pauset A, Faye PA, Prouzet-Mauleon V, Pyromali I, Nizou A, Miressi F, Sturtz F, Favreau F, Turcq B, Lia AS. Biomedicines. 2024 Jul 12;12(7):1550. doi: 10.3390/biomedicines12071550. PubMed
Functional rescue of F508del-CFTR through revertant mutations introduced by CRISPR base editing. Carrozzo I, Maule G, Gentile C, Umbach A, Ciciani M, Guidone D, De Santis M, Petris G, Vicente Galietta LJ, Arosio D, Cereseto A. Mol Ther. 2025 Mar 5;33(3):970-985. doi: 10.1016/j.ymthe.2025.01.011. Epub 2025 Jan 10. PubMed
Precise Correction of the Pde6b-L659P Mutation Causing Retinal Degeneration with Minimum Bystander Editing by Advanced Genome Editing Tools. Liu Z, Chen S, Sun Y. Research (Wash D C). 2025 Jul 2;8:0770. doi: 10.34133/research.0770. eCollection 2025. PubMed

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