CiDr-VSP L223F/C302S-mCherry pcDNA3.1 (eVSP CSmutant)
(Plasmid
#140893)
-
PurposeExpresses modified Danio rerio VSP without phosphatase activity (inactive mutant of enhanced VSP) fused with mCherry in mammalian cel
-
Depositing Lab
-
Depositing OrganizationThe University of Osaka
Located in Japan -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 140893 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 140893-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 140893-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
-
Vector backbonepcDNA3.1(-)
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameCiDr-VSP L223F/C302S-mCherry
-
SpeciesD. rerio (zebrafish); Ciona intestinalis
-
Insert Size (bp)2433
-
Mutationchanged 223L to F in Dr-VSP (zebrafish TPTE), changed 302C to S in Dr-VSP (zebrafish TPTE)
-
GenBank IDNM_001025458.1 NM_001033826.1
-
Entrez Genevsp
-
Entrez Genetpte (a.k.a. tpip, vsp, wu:fd20e11, wu:fi24b06)
-
Tag
/ Fusion Protein
- mCherry
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (unknown if destroyed)
- 3′ cloning site HindIII (unknown if destroyed)
- 5′ sequencing primer T7
- (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 140893-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 140893-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
CiDr-VSP L223F/C302S-mCherry pcDNA3.1 (eVSP CSmutant) was a gift from Yasushi Okamura (Addgene plasmid # 140893 ; http://n2t.net/addgene:140893 ; RRID:Addgene_140893) -
For your References section:
Engineering an enhanced voltage-sensing phosphatase. Kawanabe A, Mizutani N, Polat OK, Yonezawa T, Kawai T, Mori MX, Okamura Y. J Gen Physiol. 2020 May 4;152(5). pii: 133870. doi: 10.1085/jgp.201912491. 10.1085/jgp.201912491 PubMed 32167537
