E. coli expression: Z-lock cofilin dark state mutant
(Plasmid #141135)

• Purpose: a control for optogenetic cofilin analog
• Depositing Lab: Klaus Hahn
• Publication: Stone et al Nat Chem Biol. 2019 Dec;15(12):1183-1190. doi: 10.1038/s41589-019-0405-4. Epub 2019 Nov 18.

Backbone

• Vector backbone: pET
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Zdk2, Cofilin, LOV2
• Species: R. norvegicus (rat)
• Mutation: D3 deletion, I32F in Zdk2

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: universal T7 forward
• 3′ sequencing primer: universal T7 reverse

Resource Information

• Supplemental Documents:
  • 1929.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There is a point mutation in the Zdk portion of the dark control construct. In our hands, this had no effect on the experiments, and it is a position where it would not be expected to impact binding or optogenetic response.