Mammalian expression: Z-lock cofilin dark state mutant
(Plasmid
#141136)
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Purposea control for optogenetic cofilin analog
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Depositing Lab
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Depositing OrganizationUniversity of North Carolina at Chapel Hill
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 141136 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 141136-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 141136-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepEGFP
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameZdk2, Cofilin, LOV2
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SpeciesR. norvegicus (rat)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer universal cmv forward
- 3′ sequencing primer universal SV40 poly(A) reverse
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 141136-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 141136-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Mammalian expression: Z-lock cofilin dark state mutant was a gift from Klaus Hahn (Addgene plasmid # 141136 ; http://n2t.net/addgene:141136 ; RRID:Addgene_141136) -
For your References section:
Optogenetic control of cofilin and alphaTAT in living cells using Z-lock. Stone OJ, Pankow N, Liu B, Sharma VP, Eddy RJ, Wang H, Putz AT, Teets FD, Kuhlman B, Condeelis JS, Hahn KM. Nat Chem Biol. 2019 Dec;15(12):1183-1190. doi: 10.1038/s41589-019-0405-4. Epub 2019 Nov 18. 10.1038/s41589-019-0405-4 PubMed 31740825
Map uploaded by Addgene staff.
