|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14120||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6753
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameN19 Human RhoA
Alt nameDominant Negative RhoA Mutant
SpeciesH. sapiens (human)
Insert Size (bp)900
MutationSite directed mutagenesis of Thr to Asn at Codon 19
Entrez GeneRHOA (a.k.a. ARH12, ARHA, RHO12, RHOH12)
/ Fusion Protein
- Myc (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer pLXSN 5' (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byMarc Symons North Shore LIJ Res Inst Oncol and Cell Biol 350 Commnity Drive Manhasset, NY 11030
Terms and Licenses
Qiu, R.-G., Chen, J., Kirn, D., McCormick, F., & Symons, M. Nature 374, 457-459 (1995), Qiu, R.-G., Chen, J., McCormick, F., & Symons, M. Proc. Natl. Acad. Sci. USA 92, 11781-11785 (1995).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMIG-N19RhoA was a gift from Richard Hynes (Addgene plasmid # 14120 ; http://n2t.net/addgene:14120 ; RRID:Addgene_14120)
For your References section:Genomic analysis of metastasis reveals an essential role for RhoC. Clark EA, Golub TR, Lander ES, Hynes RO. Nature. 2000 Aug 3. 406(6795):532-5. 10.1038/35020106 PubMed 10952316