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(Plasmid #14120)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 14120 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    N19 Human RhoA
  • Alt name
    Dominant Negative RhoA Mutant
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    Site directed mutagenesis of Thr to Asn at Codon 19
  • GenBank ID
  • Entrez Gene
    RHOA (a.k.a. ARH12, ARHA, EDFAOB, RHO12, RHOH12)
  • Tag / Fusion Protein
    • Myc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pLXSN 5'
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Marc Symons North Shore LIJ Res Inst Oncol and Cell Biol 350 Commnity Drive Manhasset, NY 11030

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Qiu, R.-G., Chen, J., Kirn, D., McCormick, F., & Symons, M. Nature 374, 457-459 (1995), Qiu, R.-G., Chen, J., McCormick, F., & Symons, M. Proc. Natl. Acad. Sci. USA 92, 11781-11785 (1995).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMIG-N19RhoA was a gift from Richard Hynes (Addgene plasmid # 14120 ; ; RRID:Addgene_14120)
  • For your References section:

    Genomic analysis of metastasis reveals an essential role for RhoC. Clark EA, Golub TR, Lander ES, Hynes RO. Nature. 2000 Aug 3. 406(6795):532-5. 10.1038/35020106 PubMed 10952316