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pPZP FLIPmal-25u
(Plasmid #14476)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 14476 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRT100/pPZP312
  • Vector type
    Plant Expression ; Plant Nanosensor
  • Selectable markers
    Basta

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    FLIPmal
  • Alt name
    FLIPmalE W230A
  • Alt name
    MBP
  • Species
    E. coli
  • Insert Size (bp)
    2700
  • Mutation
    W230A
  • Tags / Fusion Proteins
    • ECFP (N terminal on insert)
    • EYFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer N/A
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

For more information, see
http://carnegiedpb.stanford.edu/research/frommer/nanosensors/index.html

FLIPmalEW230A with promoter from pRT100.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPZP FLIPmal-25u was a gift from Wolf Frommer (Addgene plasmid # 14476 ; http://n2t.net/addgene:14476 ; RRID:Addgene_14476)
  • For your References section:

    Rapid Metabolism of Glucose Detected with FRET Glucose Nanosensors in Epidermal Cells and Intact Roots of Arabidopsis RNA-Silencing Mutants. Deuschle K, Chaudhuri B, Okumoto S, Lager I, Lalonde S, Frommer WB. Plant Cell. 2006 Sep . 18(9):2314-25. 10.1105/tpc.106.044073 PubMed 16935985