pEF myc Gal CDK9 (P#1475)
(Plasmid #14631)

• Depositing Lab: Matija Peterlin
• Publication: Taube et al Mol Cell Biol. 2002 Jan . 22(1):321-31.

Backbone

• Vector backbone: pEF-BOS-myc
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: cdk9
• Alt name: cyclin dependent kinase 9
• Species: H. sapiens (human)
• Entrez Gene: CDK9 (a.k.a. C-2k, CDC2L4, CTK1, PITALRE, TAK)
• Tags / Fusion Proteins:
  • myc (N terminal on backbone)
  • Gal (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: EF1a forward primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Gal-Cdk9 expresses Cdk9 fused to the DBD of Gal4 and inserted at the BamHI/XbaI sites of pEF-Bos-myc(EF).