pRK5 BARK1 minigene
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14695||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4700
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameBARK1 minigene
Alt namebeta ARK1
Alt namebeta adrenergic receptor kinase
SpeciesB. taurus (bovine)
MutationBARK1 amino acids 495-689
Entrez GeneGRK2 (a.k.a. ADRBK1)
/ Fusion Protein
- 3'UTR of human b-globin (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer Sp6 (Common Sequencing Primers)
The cDNA encoding bovine BARK1 residues Gly495 to Leu689 was amplified using PCR to construct a minigene casette with newly engineered 5' and 3' ends (see Author's map). The 5' end contained an EcoRI site for subcloning, followed by the kozak sequence 5'-GCCGCCACCATGG-3'. A bclI site was inserted at the 3' end immediately following the stop codon. The final BARK1 minigene construct contains the 3'UTR of the human beta-globin gene and a polyadenylation signal.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRK5 BARK1 minigene was a gift from Robert Lefkowitz (Addgene plasmid # 14695 ; http://n2t.net/addgene:14695 ; RRID:Addgene_14695)
For your References section:Cellular expression of the carboxyl terminus of a G protein-coupled receptor kinase attenuates G beta gamma-mediated signaling. Koch WJ, Hawes BE, Inglese J, Luttrell LM, Lefkowitz RJ. J Biol Chem. 1994 Feb 25. 269(8):6193-7. PubMed 8119963
Map uploaded by the depositor.