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pET-FleBt
(Plasmid #149302)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 149302 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET28a
  • Backbone manufacturer
    EMD Biosciences
  • Backbone size (bp) 5369
  • Modifications to backbone
    insertion of FleBt solubility tag
  • Vector type
    Bacterial Expression
  • Promoter T7
  • Tags / Fusion Proteins
    • FleBt (N terminal on insert)
    • HRV 3C protease recognition site (N terminal on insert)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    BL21 (DE3) strain should be used for expression. Protein induction at lower temperature
  • Copy number
    High Copy

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-FleBt was a gift from Michael Kolbe (Addgene plasmid # 149302 ; http://n2t.net/addgene:149302 ; RRID:Addgene_149302)

  • For your References section:

    Flagellin lysine methyltransferase FliB catalyzes a [4Fe-4S] mediated methyl transfer reaction. Wang C, Nehls C, Baabe D, Burghaus O, Hurwitz R, Gutsmann T, Broring M, Kolbe M. PLoS Pathog. 2021 Nov 17;17(11):e1010052. doi: 10.1371/journal.ppat.1010052. eCollection 2021 Nov. 10.1371/journal.ppat.1010052 PubMed 34788341
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